bak rabbit polyab Search Results


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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
Bak Rabbit Polyab, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Proteintech cyclin b1 rabbit polyab antibody
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Proteintech c myc rabbit polyab
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
Cyclin D1 Mouse Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech hrp conjugated gapdh mab
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Cell Signaling Technology Inc bak1
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
Bak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc 147 anti pcaf
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Cell Signaling Technology Inc anti creb
Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic <t>proteins</t> <t>Bad,</t> Bcl-2, Bcl-xl and <t>Bak,</t> and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
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Image Search Results


Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic proteins Bad, Bcl-2, Bcl-xl and Bak, and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control

Journal: BMC Microbiology

Article Title: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway

doi: 10.1186/s12866-016-0842-0

Figure Lengend Snippet: Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic proteins Bad, Bcl-2, Bcl-xl and Bak, and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control

Article Snippet: The antibodies used in the present study included rabbit anti-SOD2, rabbit anti-RRAS, rabbit anti-RAF1, rabbit anti-ERK1/2, rabbit anti-BAK, rabbit anti-BAD, rabbit anti-BCL2, rabbit anti-Caspase 3, rabbit anti-Caspase 9, rabbit anti-Cytochrome c (Cyt-C) (Proteintech Group, Campbell Park, Chicago, USA), rabbit anti-phosphorylated Raf1 (p-Raf1), rabbit anti-MEK1/2, rabbit anti-phosphorylated MEK1/MEK2 (p-MEK1/2), rabbit anti-phosphorylated ERK1/2 (p-ERK1/2) (Signalway Antibody, MD, USA), rabbit anti-GSS (ABGENT, San Diego, USA) and mouse anti-β-actin.

Techniques: Expressing, Infection, Cell Culture, Western Blot