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Image Search Results
Journal: BMC Microbiology
Article Title: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway
doi: 10.1186/s12866-016-0842-0
Figure Lengend Snippet: Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic proteins Bad, Bcl-2, Bcl-xl and Bak, and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in ( a ) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control
Article Snippet: The antibodies used in the present study included rabbit anti-SOD2, rabbit anti-RRAS, rabbit anti-RAF1, rabbit anti-ERK1/2,
Techniques: Expressing, Infection, Cell Culture, Western Blot